Coated wire stands or cardboard holders available for use with autoclave bags
Autoclave bags are impermeable to steam, and for this reason should not be twisted or taped shut as this could prevent the load from being sterilized correctly
Do not overload the autoclave. Leave plenty of room for thorough steam circulation
Autoclave bags containing biological waste should be autoclaved for a minimum of 30 minutes at 121°C to ensure sterilization.
Use high temperature bags and autoclave at 135°C for a minimum of 30 minutes for the decontamination and inactivation of particularly resistant biological waste* (* These are purely guidelines — times and temperatures will be dependent on the nature of the autoclave load.)
Water should be added to bags of solid waste to allow effective sterilization of the load
Do not put sharp objects such as broken glassware into an autoclave bag as this can lead to puncture holes