NutriStem® hPSC XF Medium is a widely published, defined, xeno-free, serum-free cell culture medium designed to support the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells. Protocols have been established around the world for applications ranging from derivation to differentiation. NutriStem® hPSC XF Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, superior cell attachment and proliferation is observed with NutriStem® hPSC XF Medium aid high-throughput screening applications. NutriStem® hPSC XF Medium exhibits a consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation as well as increased reproducibility shown in long-term growth of over 50 passages.
Low-protein formulation that contains stable L-alanyl-L-glutamine and HSA.
NutriStem® hPSC XF features
Defined, serum-free, and xeno-free
Flexible and compatible with multiple matrices
Amenable to weekend-free culture
FDA Drug Master File (DMF) available, produced under cGMP
Enables efficient expansion and growth of hES and hiPS cells in feeder-free culture systems
Extensively tested and widely used on multiple hES and iPS cell lines, including H1, H9.2, I6, I3.2, and CL1
Store at -20ºC
NutriStem® hPSC is routinely tested for optimal maintenance and expansion of undifferentiated hESCs. Additional standard evaluations are pH, osmolality, endotoxins and sterility tests.
Instructions for Use
Upon thawing, the medium may be stored at 2 - 8ºC for 10 to 14 days. Media should be aliquotted into smaller working volumes to avoid repeated freeze/thaw cycles. Avoid exposure to light. Note: A common feeder-free basement membrane matrix is Matrigel, which is not xeno-free. Effective xeno-free alternatives to Matrigel is recombinant laminin, such as LaminStem(R) 521 (BI Cat. No. 05-753-1F) which has been validated to successfully culture human ES and iPS cells using NutriStem® hPSC XF medium.
NutriStem® hPSC XF is registered as an In-vitro diagnostic (IVD) medical device. NutriStem® is a registered trademark of Biological Industries. A Drug Master File (DMF) for NutriStem® hPSC XF is available.