MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium support long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.
hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media as well as other commercial serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles and karyotypic stability over long-term culture.
Serum-free, xeno-free, defined culture system
Superior cell growth compared to serum-containing media
Supports long-term growth and self-renewal of hMSCs from multiple sources
Consistent, reliable MSC cultures
Eliminates the need to spend time and money prequalifying FBS lots
GMP-defined, FDA Drug Master File (DMF) available
Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.
MSC NutriStem® XF Medium is application tested and validated for optimal maintenance and expansion of undifferentiated hMSCs, while maintaining their multi-lineage differentiation potential. Additional QC validation includes proper pH, osmolality, endotox
The complete mesenchymal stem cell culture media consist of two components: MSC NutriStem® XF Basal Medium (05-200-1) and MSC NutriStem® XF Supplement Mix (05-201-1). Individual components are not sold separately.
Instructions for Use
Storage and Stability: MSC NutriStem® XF Basal Medium (05-200-1) should be stored at 2 to 8°C. MSC NutriStem® XF Supplement Mix (05-201-1) should be stored at -20°C. Avoid freeze/thaw cycles. The complete MSC NutriStem® XF Medium is stable at 2 to 8°C for up to 30 days. Avoid exposure to light. Medium Preparation: Thaw frozen MSC NutriStem® XF Supplement Mix at at 2 to 8°C or room temperature. MSC NutriStem® XF Basal Medium contains L-glutmine. To prepare 100 mL of complete medium: aseptically add 0.6 mL of MSC NutriStem® XF Supplement Mix to 100 mL of MSC NutriStem® XF Basal Medium To prepare 500 mL of complete medium: aseptically add 3 mL of MSC NutriStem® XF Supplement Mix to 500 mL of MSC NutriStem® XF Basal Medium. Store complete MSC NutriStem® XF Medium at 2 to 8°C protected from light for up to 30 days.
MSC NutriStem® XF Basal Medium is registered as an in-vitro diagnostic (IVD) medical device. A Drug Master File (DMF) for MSC NutriStem® XF is available.