E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.
All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.
DHB – Defibrinated Horse Blood
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of horse blood are not identical to sheep blood and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa.
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